The present invention relates to methods and kits for isolation of cell-derived vesicles and their associated macromolecules like nucleic acids, proteins, lipids 5 metabolites etc. from one or more blood, serum, plasma, saliva, urine, cerebrospinal fluid, breast milk, tear, conditioned culture media etc. to assist detection, prevention, and understanding of disease biology. The invention offers various advantages including simple technical solutions which are cost-effective, time-saving and scalable for large industrial outputs. 10 [To be published with Figure 1A]
