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RAPID AND SENSITIVE DETECTION METHOD FOR THE PHOSPHODIESTERASE TYPE 5 INHIBITORS USING HIGH-TEMPERATURE GAS CHROMATOGRAPH CAPILLARY COLUMN
专利权人:
REPUBLIC OF KOREA (SUPREME PUBLIC PROSECUTOR'S OFFICE)
发明人:
KIM, JIN YOUNG,KWON, WOON YONG,SUH, SUNG ILL,IN, MOON KYO
申请号:
KR20130119801
公开号:
KR101421100(B1)
申请日:
2013.10.08
申请国别(地区):
韩国
年份:
2014
代理人:
摘要:
The present invention relates to a method to rapidly and sensitively detect erection inducers such as sildenafil, tadalafil, and vardenafil. The method comprises the steps of: extracting erection inducer components such as sildenafil, tadalafil, and vardenafil from pharmaceutical formulations including erection inducer components such as sildenafil, tadalafil, and vardenafil using ultrasonic waves and an organic solvent; centrifuging and refining the extracted components obtained in the extracting step using an ultrahigh-speed centrifuging device; separating the refined extract using a high temperature gas phase chromatograph analyzer including a capillary separation pipe; and measuring component contents of the compound separated by a high temperature gas phase chromatography means using a mass spectrometer through an electron impact ionization process. By means of the present invention, sildenafil, tadalafil, and vardenafil can effectively be extracted and refined from various pharmaceutical formulations, a
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