Chemoenzymatic glycoengineering of antibodies and Fc fragments thereof

• 收藏
• 分享

专利权人:

UNIVERSITY OF MARYLAND; BALTIMORE

发明人:

Lai-Xi Wang,Wei Huang

申请号：

US15843160

公开号：

US10344063B2

申请日:

2017.12.15

申请国别(地区):

US

年份:

2019

代理人:

摘要：

The present invention provides for recombinant Endo-S mutants that exhibit reduced hydrolysis activity and increased transglycosylation activity for the synthesis of glycoproteins wherein a desired sialylated oxazoline or synthetic oligosaccharide oxazoline is added to a core fucosylated or nonfucosylated GlcNAc-protein acceptor. Such recombinant Endo-S mutants are useful for efficient glycosylation remodeling of IgG1-Fc domain to provide different antibody glycoforms carrying structurally well-defined Fc N-glycans.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/