PURPOSE: A prefabricated unit panel for a house structure is provided, which always keeps an inner temperature of the house structure at proper degree with small energy by isolating heat-transmission between the indoor and the outdoor of the house structure. And a construction method of house structure using the same is also provided. CONSTITUTION: The prefabricated unit panel for a house structure comprises: (i) a square frame body part(10) which comprises an upper and lower part boards(11) and a left and right side boards(12)(13), wherein many air inlets(12a) and air outlets(13a) are formed on one of the left and right side boards; (ii) a pair of one side and the other side cover parts(20) which are fixed on both sides of the frame body part to be disassembled or assembled. The method comprises steps of: (i) installing vertical frames and horizontal frames on a position to construct the house structure at regular intervals; (ii) installing a bottom rail on the outside bottom of the vertical frames and the h