A method for controlling obesity by using Antrodia camphorata is disclosed. A composition of Antrodia camphorata prepared from dried fruiting bodies of Antrodia camphorata is used to reduce lipid accumulation in adipocytes for controlling obesity, and the method includes steps of: providing a composition of Antrodia camphorata prepared from dried fruiting bodies of Antrodia camphorata and applying the composition to reduce lipid accumulation for improving symptoms of obesity.