The method this invention including the fact that the cell is cultured in the nutrient medium which includes the serum which removes the heparin adsorption component, inducing the differentiation of the internal organs fat fore-quarter cell and the method of culturing the internal organs fat cell are offered. In addition as for this invention, for example, the serum is applied to the heparin affinity column, the method of removing the heparin adsorption component by making the heparin adsorption component the heparin adsorb with this is offered. The internal organs fat cell which was cultured by the method of this invention, in research of differentiation, growth and metabolism of the fat cell, in emergence of disease such as diabetes, high fat blood symptom, hypertension and arteriosclerosis and elucidation of mechanism of advance, and is useful in development of the medicine in order it prevents and to remedy that kind of disease and.本発明は,ヘパリン吸着成分を除去した血清を含む培地中で細胞を培養することを含む,内臓脂肪前駆細胞の分化を誘導する方法および内臓脂肪細胞を培養する方法を提供する。本発明はまた,例えば,血清をヘパリンアフィニティーカラムに適用し,このことによりヘパリン吸着成分をヘパリンに吸着させることによりヘパリン吸着成分を除去する方法を提供する。本発明の方法により培養された内臓脂肪細胞は,脂肪細胞の分化,成長および代謝の研究に,糖尿病,高脂血症,高血圧症および動脈硬化等の疾病の発症および進行のメカニズムの解明に,およびそのような疾病を予防および治療するための薬剤の開発に有用である。
