The present invention relates to a composition for nucleus-independent, sustained inhibition of gene expression, comprising an mRNA fragment of T7 RNA polymerase, plasmid DNA for self-amplification of the T7 RNA polymerase, and a DNA fragment encoding a gene expression inhibitor； a transporter of the gene expression inhibitor comprising the composition； and a method of preparing the transporter.The composition of the present invention and the liposome transporter of the present invention comprising the composition can improve the expression of shRNA in the cytoplasm through self-amplification of nucleus-independent, sustained self-amplification of T7 RNA polymerase, and deliver them in a cancer tissue-specific manner. Therefore, the composition and the liposome transporter of the present invention can be utilized for use in treating chronic diseases that require reduced frequency of administration and long-term inhibition of gene expression.
