Disclosed are a minimally invasive surgical instrument and a method for knotting thereby. The minimally invasive surgical instrument is formed by a first clamp handle (11) and a second clamp handle (12) intersecting at a connection point, and has a clamping head (2) as the head end and a handle (3) as the tail end for opening or closing the clamping head. The end portion of the head end of the second clamp handle (12) has a mantle line part (4). When the first clamp handle (11) and the second clamp handle (12) are closed, the mantle line part (4) and the head end (111) of the first clamp handle (11) abut each other. A wave sheet (5) is connected to the inner side of the head end (111) of the first clamp handle (11), and a crack (7) is formed between the wave sheet (5) and the first clamp handle (11) and is capable of accommodating a suture line. The fixed end of the wave sheet (5) is connected to the first clamp handle (11), and the free end (51) of the wave sheet (5) leans against the mantle line part (4), and moves as the first clamp handle (11) moves without being restricted by the mantle line part (4). After using this minimally invasive surgical instrument for knotting in vitro, the knot is delivered to the suture in the body. This method has a high success rate for knotting, and can conveniently and efficiently complete knotting operations, reduces the number of surgical instruments used, and will help improve the operation efficiency.Linvention concerne un instrument chirurgical mini-invasif et un procédé associé permettant de nouer. Linstrument chirurgical mini-invasif est formé par une première poignée de serrage (11) et une seconde poignée de serrage (12) se croisant au niveau dun point de raccordement, et comporte une tête de serrage (2) en tant quextrémité de tête et une poignée (3) en tant quextrémité de queue pour louverture ou la fermeture de la tête de serrage. La partie dextrémité de lextrémité de tête de la seconde poignée de serrage (12) possède u
