Disclosed are a minimally invasive surgical instrument for knotting and a method for knotting and trimming thereof. The minimally invasive surgical instrument is formed by a first clamp handle (11) and a second clamp handle (12) intersecting at a connection point, and has a clamping head (2) as the head end and a handle (3) as the tail end for opening or closing the clamping head the end portion of the head end (121) of the second clamp handle has a mantle line part (4) a wave sheet (5) is connected to the inner side of the head end (111) of the first clamp handle, and a crack (7) is formed between the wave sheet and the first clamp handle and is capable of accommodating a suture line a trimming part (8) is provided on the first clamp handle and the second clamp handle, and winding hooks (9A, 9B) are fixed at both side of the trimming part on a fixed portion (9) on the first clamp handle and the second clamp respectively. After using this minimally invasive surgical instrument for knotting in vitro, the knot is delivered to the suture in the body, and after tightening the knot, the suture line is trimmed by the trimming part. This method has a high success rate for knotting, and can conveniently and efficiently complete knotting operations, gathers the knotting and suture line trimming functions into one, reduces the number of surgical instruments used, and will help improve the operation efficiency.Linvention concerne un instrument chirurgical mini-invasif pour nouer et un procédé associé permettant de nouer et de couper. Linstrument chirurgical mini-invasif est formé par une première poignée de serrage (11) et une seconde poignée de serrage (12) se croisant au niveau dun point de raccordement, et comprend une tête de serrage (2) en tant quextrémité de tête et une poignée (3) en tant quextrémité de queue pour louverture ou la fermeture de la tête de serrage la partie dextrémité de lextrémité de tête (121) de la seconde poignée de serrage a une broche à fil (4) un r
