The present invention relates to a method for preparing a chitosan-based matrix (4) comprising the following steps:- a) two separate chitosan porous layers (1, 2) obtained from lyophilisation of layers of one or more solutions of chitosan are provided,- b) the chitosan porous layers of a) are neutralized and washed,- c) a face (1a, 2a) of each chitosan porous layer is acidified with an acid,- d) a fiber reinforcement member (3) is provided and positioned between the two acidified faces of the two chitosan porous layers,- e) the two chitosan porous layers and the fiber reinforcement member are let to dry to obtain a matrix having the fiber reinforcement member embedded therein.The invention also relates to an implant comprising a matrix obtained according to such a method.
