RID Tomas D. (US),РИД Томас Д. (US),BICh Robert P. (US),БИЧ Роберт П. (US),RID TOMAS D.,РИД Томас Д.,BICH ROBERT P.,БИЧ Роберт П.
申请号：
RU2009103208/15
公开号：
RU0002468820C2
申请日:
2007.07.26
申请国别(地区):
RU
年份:
2012
代理人:
摘要：
FIELD: medicine.SUBSTANCE: group of inventions refers to medicine, more specifically to oncology and concerns methods of treating or personalising therapy of cancer in a subject by specific removal of malignant cells from a cell population of peripheral blood, as well as a such method for specific removal of malignant cells from a cell population of peripheral blood intended for autologous transplantation. A cell population of peripheral blood containing malignant and non-malignant cells is taken from a subject and analysed for activity of at least one cancer marker in said cell population. It is followed by introducing into the cells a polynucleotide coding the selected marker and a polypeptide lethal for said malignant cells wherein expression of the lethal polypeptide is under direct or indirect control of a cancer marker promoter. The cells are exposed in selection conditions to produce cells containing said polynucleotide. The produced cells are treated in the conditions of expression induction of the lethal polypeptide which kills the cells expressing the cancer marker. Then the killed cells are separated from the living cells to be returned to the subject body.EFFECT: presented new method for specific removal of malignant cells enables both cancer cell reinfusion into the patient's body in autologous transplantation, and makes it possible to personalise therapy of a malignant disease in the subject by structuring an individual therapeutic polynucleotide depending on subject's genotype.45 cl, 8 dwg, 5 exГруппа изобретений относится к медицине, конкретно к онкологии, и касается способов лечения или индивидуализации лечения рака у субъекта путем специфичного удаления злокачественных клеток из популяции клеток периферической крови, а также как такового способа специфичного удаления злокачественных клеток из популяции клеток периферической крови, предназначенной для аутотрансплантации. Получают популяцию клеток периферической крови, содержащую злокачественные и
