Method for the microbiological cleaning of patient and locker rooms, living spaces in hospitals, retirement and nursing homes, the method consisting of a first step, being the automatic atomization (3) of a biocide (2) that decontaminates and a following second step, being the atomization (3) of a benign biological culture (4) of benign aerobic spore formers for biological stabilization, whereby the atomization in both steps delivers microdrops of 5 to 10 microns.