Abstract of invention (Not applicable in case of provisional specification. Separate sheet to be used for this column.)e antiurolithiatic activity of a polyherbal formulation, Unex. Urolithiasis or form The present invention relates to thation of urinary calculi at any level of the urinary tract is a common condition and are characterized clinically by colicky pain. The present invention relates with the discovery of one polyherbal product, Unex manufactured by Unijules Life Sciences Ltd., and Associated Companies, Nagpur, containing the extracts of Boerrhavia diffusa and Tribulus terrestris in the ratio of 2:1, which has been found effective in prevention of formation of kidney stones. The capsules containing the extracts of both the herbs were procured from the Unijules Life Sciences Ltd., and Associated Companies, Nagpur. A study was conducted to determine the effect of Unex in kidney stone formation using Ethylene glycol induced model. Cystone 750 mg/kg was used as the standard drug to compare the efficacy of Unex. Five groups containing 6 animals in each group were subjected to 0.75% ethylene glycol into drinking water for four weeks and the sixth group was kept as normal control. 15" day onwards till 28! day Unex in two doses, 200 and 400 mg/kg, and cystone were given to three groups of animals. Remaining one group served as negative control, untreated. On 29th day the urine of animals was analyzed for calcium, phosphate and oxalate. Serum was analyzed for creatinine, uric acid and urea nitrogen. Urinary volume was measured by using measuring cylinder, and reported in ml. Uric acid crystals were found to deposit most frequently in the concentrated acid urine. Thus the acidity of the urine was also tested using pH meter. Results indicated that the capsule Unex prevented the rise of Oxalate, calcium and phosphate which are the main markers of urolithiasis (Table 1). Stone formation leads to the increased levels of blood urea nitrogen, creatinine and uric acid.
