Methods, systems, and computer readable media for synchronizing timing among network interface cards in a network equipment test device are disclosed. One method includes generating and sending timing synchronization measurement signals to the management network interface card and a test network interface card in the network equipment test device. The method further includes recording times of receipt of the timing synchronization measurement signals at the management network interface card and the test network interface card. The method further includes adjusting timing of the test network interface card using the times of receipt of the timing synchronization measurement signals.
