CHIKOBAVA, Merab Georgievich;RUBALSKY, Evgeny Olegovich;ЧИКОБАВА, Мераб Георгиевич;СИНИЦА, Александр Владимирович;РУБАЛЬСКИЙ, Евгений Олегович;SINITSA, Alexandr Vladimirovich; Evgeny Olegovich;RUBALSKY;RUBALSKY, Oleg Vasilievic
发明人:
RUBALSKY, Evgeny Olegovich,РУБАЛЬСКИЙ, Евгений Олегович,CHIKOBAVA, Merab Georgievich,ЧИКОБАВА, Мераб Георгиевич,SINITSA, Alexandr Vladimirovich,СИНИЦА, Александр Владимирович,RUBALSKY, Oleg Vasilievic
申请号：
RURU2016/000859
公开号：
WO2017/116282A1
申请日:
2016.12.09
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
The invention relates to microbiology and can be used in biotechnology for producing a product containing bacteriophages. According to the present method, 30-120 minutes after commencement of cultivation at an optimal temperature for the growth of a culture of a fast-growing host strain, swabs are made every 30-60 minutes from the host strain culture from the surface of a solid growth medium or from a liquid culture medium, the swabs are dyed with a solution of acridine orange in a final concentration of from 0.001% to 0.02% or with a solution of acridine yellow in a final concentration of from 0.01% to 0.2%, a dyed swab is examined under a fluorescence microscope and the time interval for inoculation with stock bacteriophage is set according to when the proportion of acridine orange-dyed cells fluorescing shades of orange or red, or the proportion of acridine yellow-dyed cells fluorescing shades of yellow or orange, reaches at least 50% in relation to the total quantity of host strain cells in the dyed swab, and at least 10% of the total number of host strain cells is comprised of cells with non-uniform fluorescence, and more particularly: paired cells adjoined by their poles and being shaped like rods with non-uniform fluorescence of the cytoplasm, or spherical and ellipsoidal cells with a weakly fluorescing central cross section. The technical result of the invention is that of providing a consistently high titre of bacteriophage during production of a phage lysate under conditions that permit adjustment of growth medium recipes and a greater range of variability of the host strain and bacteriophage cultivation parameters.L'invention se rapporte au domaine de la microbiologie et peut être utilisée dans les biotechnologies pour obtenir des produits contenant des bactériophages. Selon le procédé, au bout de 30-120 minutes après le lancement de la culture à une température optimale pour la croissance d'une culture de souches hôtes à croissance rapide, on prélève tou
