Brown, Jeffrey A.,Song, Hee-Sook,Roche, Christina E.,Lai, Fang-Ming
申请号：
AU2008257506
公开号：
AU2008257506B2
申请日:
2008.05.30
申请国别(地区):
AU
年份:
2013
代理人:
摘要：
The present invention relates to a method for excising a nucleic acid sequencefrom the genome of a plant or a plant cell. This method is based on the steps of transforminga plant cell with a construct encoding a DNA double strand break inducingenzyme(DSBI), generating a transgenic plant line, performing a transient assay toanalyze the functionality of the transgenic enzyme, crossing the plant linewith a line containing a nucleic acid sequence to be excised and performing animmature embryo conversion or a tissue culture regeneration through callusformation. The method can also be reversed, which means that a plant cell is transformedwith a construct encoding a nucleic acid sequence to be excised, and the crossingis performed witha plant line containing a DSBI. As an alternative to the crossingstep, a re-transformation of a transgenic plant line with a second constructcan also be performed. The invention is also directed to a plant obtained by thismethod, or progeny, propagation material, part, tissue, cell or cell culture,derived from such a plant. Finally, the invention relates to the use of a plantor progeny, propagation material, part, tissue, cell or cell culture, derivedfrom this method, as aliment, fodder or seeds or for the production of pharmaceuticalsor chemicals.
