The present invention relates to a method of manufacturing a patty by using a mushroom concentrate and a culture medium. The method of the present invention includes: a seed making step of making seeds having a granular size of 1,500-2,500 μm through repeated fluidization while spraying a culture medium, which is made by culturing and increasing muscular satellite cells extracted from the muscular tissue of a cow, like a bottom spray under a fluidized bed coating condition； a granule making step of preparing a mushroom concentrate as a coating spray solution and inserting the seeds into a fluidized bed coater, and then, making granules, containing 30-70 wt% of beef powder, by coating the seeds with the coating spray solution through repeated fluidization while spraying the solution like a bottom spray under the fluidized bed coating condition； a mixing step of making a patty composition by mixing the granules with liquefied edible fat； and a forming step of making a patty by forming the patty composition in a patty mold and then hardening the composition. According to the present invention, in general, consumers do not have repulsion to a hamburger patty made of minced meat, and accordingly, a culture medium is made by culturing muscular satellite cells extracted from muscular cells of a cow, or culture meat is formed by increasing and dividing the culture medium into muscular cells, but culture meat before the formation of muscular fibers is used for a better taste and mouthfeel.본 발명은 버섯농축액과 배양액을 이용한 패티 제조 방법에 관한 것이다.본 발명의 버섯농축액과 배양액을 이용한 패티 제조 방법은, 소의 근육 조직으로부터 추출된 근육 위성 세포를 배양하여 증식시킨 배양액을 유동층코팅조건하에서 바텀스프레이(bottom spray) 형태로 분무하면서 반복적인 유동화를 통해 1500-2,500 μm 입자크기의 씨드를 제조하는 씨드제조단계와； 버섯농축액을 코팅분무액으로 준비하고, 상기 씨드를 유동층코팅기 내부에 투입한 후, 상기 코팅분무액을 유동층코팅조건하에서 바텀스프레이(bottom spray) 형태로 분무하면서 반복적인 유동화를 통해 씨드에 코팅하여 쇠고기분말 함량비율이 30 내지 70중량%가 되는 구형 과립을 제조하는 과립제조단계와； 상기 구형과립에 액상의 식용 지방을 혼합 및 교반하여 패티조성물을 제조하는 혼합단계와； 상기 패티조성물을 패티용 틀에 투입하여 성형한 후 경화시켜 패티를 제조하는 성형단계；를 포함하여 구성된다.본 발명에
