PROBLEM TO BE SOLVED: To provide a separator and a separation method suitable for separating, by dielectrophoresis, electrically neutral minute substance present inside a liquid. SOLUTION: The minute substance separator 10 includes: a dielectrophoretic cell 11 for introducing a sample solution containing a substance to be separated that receives dielectrophoretic force and that is electrically neutral; at least two or more electrodes 12a, 12b that are arranged inside the dielectrophoretic cell 11 so that at least a part of electric field distribution can be uneven; and a degree of separation evaluating section 13 that, on the basis of a degree of separation and a degree of concentration, evaluates the state of separation of the substance to be separated that is separated by moving the substance in a prescribed direction in the dielectrophoretic cell 11 by actuating an electric field in the sample solution via the electrodes 12a, 12b. COPYRIGHT: (C)2011,JPO&INPIT