A preparation method of polysaccharide extract and freeze-crystal liposome comprises: using a wall disruption technique, a water-bath extraction and an alcohol precipitation to extract polysaccharide ingredient of plants or natural organisms using the phospholipid as the carrier to cover the polysaccharide by means of the film/high pressure homogenous emulsion method to prepare the polysaccharide liposome adding 10% of sucrose as the cryoprotectant to prepare the liposome freeze-crystal by lyophilization and finding the functionality of the polysaccharide freeze-crystal liposome and the optimal conditions of the functionality, thereby increasing the efficacy, functionality and safety of the freeze-crystal liposome product.一種多醣體萃取及凍晶微脂粒製備技術,將植物或天然有機體利用破壁技術及水浴萃取、酒精沈析進行多醣體成份萃取,以磷脂質作為載體,用薄膜/高壓均質乳化法包覆多醣體,製備多醣體微脂粒,再添加10%蔗糖作為抗凍劑,利用冷凍乾燥技術製備微脂粒凍晶,找出多醣體凍晶微脂粒之功能性及有功能性之最佳化條件,藉由此技術提高凍晶微脂粒產品有效性、功能性及安全性。