The present invention relates to improved NanobodiesTMagainst Tumor Necrosis Factor－alpha (TNF－alpha), as well as to polypeptides comprising or essentially consisting of one or more of such Nanobodies. The invention also relates to nucleic acids encoding such Nanobodies and polypeptides；to methods for preparing such Nanobodies and polypeptides；to host cells expressing or capable of expressing such Nanobodies or polypeptides；to compositions comprising such Nanobodies, polypeptides, nucleic acids or host cells；and to uses of such Nanobodies, such polypeptides, such nucleic acids, such host cells or such compositions, in particular for prophylactic, therapeutic or diagnostic purposes, such as the prophylactic, therapeutic or diagnostic purposes.本案係改良型對抗腫瘤壞死因子－α(TNF－α)之奈米抗體TM，其包含或本質上帶有一個以上該奈米抗體的聚胜肽有關。此本案也相關於決定該奈米抗體與聚胜肽的核酸；製備該奈米抗體與聚胜肽的方法；表現或有能力表現該奈米抗體或聚胜肽的宿主細胞；包含該奈米抗體、聚胜肽、核酸或宿主細胞的組成；及該奈米抗體、該聚胜肽、該核酸、該宿主細胞或該組成的應用，特別是針對預防、治療或診斷目的，像是預防、治療或診斷之用途。
