BICOUCHE LIPIDIQUE SUPPORTÉE AUTOSSEMBLEUSE CHARGÉE NÉGATIVEMENT SUR DES NANOPARTICULES DE SILICE MÉSOPOREUSE, PROCÉDÉ DE SYNTHÈSE ET D'UTILISATION EN TANT QUE NANOVECTEUR
Luxembourg Institute of Science and Technology (LIST)
发明人:
申请号：
EP16742296.3
公开号：
EP3324940B1
申请日:
2016.07.22
申请国别(地区):
EP
年份:
2018
代理人:
摘要：
A method for manufacturing a negatively charged supported lipid bilayer. The method comprises the steps of preparing a formulation comprising at least three lipids (1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS), cholesterol and at least one lipid different from DOPS and cholesterol) dissolved in a first solvent, of evaporating the first solvent, of adding an aqueous formulation of mesoporous silica nanoparticles, of performing an ultra-sonication and of performing a centrifugation. The method is remarkable in that the number of equivalents of cholesterol relative to one equivalent of DOPS is comprised between 2.30 and 2.70. Additionally, negatively charged supported lipid bilayer on a mesoporous silica nanoparticle comprising cholesterol, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and at least one lipid different from DOPS and cholesterol.
