The present invention relates to antisense antiviral compounds and methods of their use and production in inhibition of growth of viruses of the Orthomyxoviridae family and in the treatment of a viral infection. The compounds are particularly useful in the treatment of influenza virus infection in a mammal. Exemplary antisense antiviral compounds are substantially uncharged, or partially positively charged, morpholino oligonucleotides having 1) a nuclease resistant backbone, 2) 12-40 nucleotide bases, and 3) a targeting sequence of at least 12 bases in length that hybridizes to a target region selected from the following: a) the 5’ or 3’ terminal 25 bases of the negative sense viral RNA segment of Influenzavirus A, Influenzavirus B and Influenzavirus C b) the terminal 30 bases of the 5’ or 3’ terminus of the positive sense vcRNA c) the 45 bases surrounding the AUG start codon of an influenza viral mRNA and d) 50 bases surrounding the splice donor or acceptor sites of influenza mRNAs subject to alternative splicing.本發明係關於反義抗病毒化合物,及其在正黏病毒科(Orthomyxoviridae)病毒之生長抑制及病毒感染之治療中之使用方法及產生方法。該等化合物尤其可用於治療哺乳動物之流感病毒感染。實例性反義抗病毒化合物係實質上不帶電或部分帶正電之嗎啉基寡核苷酸,其具有1)抗核酸酶骨架,2)12-40個核苷酸鹼基,及3)至少12個鹼基長之靶向序列,與選自以下之靶區域雜交:a)流感病毒A、流感病毒B及流感病毒C之負義病毒RNA區段中5或3端之25個鹼基;b)正義vcRNA中5或3端之末端30個鹼基;c)流感病毒mRNA中AUG起始密碼子周圍之45個鹼基;及d)進行選擇性剪接之流感mRNAs中剪接供體或剪接受體位點周圍之50個鹼基。(無元件符號說明)
