PURPOSE: A method for preparing an extract containing polyhydroxylated alkaloid from a silkworm is provided to extract a polyhydroxylated alkaloid active ingredient which can be applied to a natural product novel drug and a health functional food in a substantial level.CONSTITUTION: A method for preparing an extract containing polyhydroxylated alkaloid from a silkworm comprises: a step of quickly freezing or freeze-drying and pulverizing an alive silkworm(S100) a step of adding ethanol, methanol, or a mixture thereof to the silkworm powder and extracting by stirring, maceration, sonication, or reflux to obtain an extract(S200) a step of centrifuging or filtering the extract(S300) and a step of spray-drying or freeze-drying(S400) the extract.COPYRIGHT KIPO 2013[Reference numerals] (S100) Step of quickly freezing or freeze-drying and pulverizing an alive silkworm to be powderized (S200) Step of adding ethanol, methanol, or a mixture thereof to the silkworm powder as a solvent and extracting by stirring, maceration, sonication, or reflux to obtain an extract (S300) Step of centrifuging or filtering the extract (S400) Step of spray-drying or freeze-drying the centrifuged or filtered filtrate and obtaining extract powder본 발명은 누에로부터 폴리하이드록실레이티드 알칼로이드 성분을 유효성분으로 하는 추출물 제조방법 및 그 분석방법, 추출물에 관한 것으로서, (a) 생누에를 급속냉동 또는 동결건조 후 분쇄하여 분말화하는 단계 (b) 상기 냉동누에분말 또는 동결건조누에분말을 에탄올, 메탄올 또는 이들의 혼합액을 용매로 하여 교반, 냉침, 초음파, 환류 중 어느 하나의 방법을 사용하여 추출액을 수득하는 단계 (c) 상기 추출액을 원심분리 또는 필터여과 하는 단계 및 (d) 상기 원심분리 또는 필터여과된 여액을 분무건조 또는 동결건조하여 추출물 분말을 수득하는 단계를 포함하여 구성된다.
