MANUFACTURING METHOD FOR ACTIVE MATERIALS OF ANTI-COMPLEMENT FROM SEAWEED FULVESCENS AND ACTIVE MATERIALS OF ANTI-COMPLEMENT FROM SEAWEED FULVESCENS MANUFACTURED BY THE SAME
The present invention relates to a method for preparing an anti-complement active substance derived from Meesengi and to an anti-complement active substance derived from mesentery prepared thereby. The method for preparing an anti-complement active substance derived from Maesengi according to the present invention includes washing and freeze drying steps of freeze-drying after washing Maesengi to remove foreign substances (S100)； After crushing the freeze-dried Maesengi to prepare Maesengi powder, Maesengi crushing and hot water extraction step of extracting the Maesengi powder to prepare a Maesengyi hot water extract (S200)； Hot-water extract freeze-drying step (S300) of freeze-drying the hot-water extract； Reflux extraction step (S400) of separating a methanol-soluble substance and a methanol-insoluble substance contained in the freeze-dried medium-water extract by reflux extraction of the freeze-dried medium-water extract with methanol； A precipitate freeze-drying step of dissolving the separated methanol-insoluble substance in distilled water, adding ethanol to the methanol-insoluble substance dissolved in distilled water to obtain an ethanol precipitate, and freeze-drying the ethanol precipitate to prepare a lyophilized ethanol precipitate (S500 )； A first centrifugation step (S600) of dissolving the freeze-dried ethanol precipitate in distilled water and then performing a first centrifugation to obtain a first supernatant； A second centrifugation step (S700) of adding boric acid (H 3 BO 3) to the first supernatant and then performing a second centrifugation to obtain a second supernatant； A precipitate obtaining step (S800) of obtaining a precipitate produced after adding citric acid and ethanol to the second supernatant； And a step (S900) of preparing an anti-complement active substance by dialysis of the precipitate produced by adding ethanol and then freeze-drying to obtain an anti-complement active substance. According to the above-described configuration,
