GENETIC MARKER FOR DISCRIMINATING AND DETECTING CAUSATIVE BACTERIA OF FISH EDWARDSIELLOSIS AND STREPTOCOCCOSIS, AND METHOD FOR DISCRIMINATING AND DETECTING CAUSATIVE BACTERIA USING SAME
Kim Myoung Sug,Jung Sung-Hee,Choi Hye Sung,Han Hyun-Ja,Do Jeung-Wan,Kim Jin-Do
申请号：
US201615571442
公开号：
US2018127798(A1)
申请日:
2016.12.06
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
The present disclosure relates to genetic markers for discrimination and detection of bacteria causing Edwardsiellosis and Streptococcosis in fish, and a method for discriminating and detecting the bacteria using the same. A genetic marker for discrimination and/or detection of each of Edwardsiella tarda, Streptococcus iniae, Streptococcus parauberis and Lactococcus garvieae, which cause fish diseases, is selected and a peptide nucleic acid and a primer pair, which are specific for the genetic marker, are used to amplify and obtain melting curves having different fluorescences depending on bacterial species. Thus, bacteria that cause fish diseases can be discriminated and whether or not fish would be infected with the bacteria can be detected in a simple, rapid and accurate manner.
