The invention is the products and methods associated with purifying overexpressed recombinant recombinases from a host cell line resulting in an un-tagged protein of interest without any additional, non-native amino acids. The invention employs at least one DNA vector that co-expresses a tagged fusion protein and the recomibinase protein with the recombinase protein having an affinity for binding to the the tagged fusion protein. Isolation methods of the recominbase protein include the targeting of the tagged fusion protein.
