Provided is a three-dimensional printing composition including decellularized extracellular matrix and riboflavin as a crosslinking agent. A three-dimensional structure having high mechanical strength can be prepared by performing a printing process using the three-dimensional printing composition according to the present invention and a layer-by-layer process through crosslinking under UVA light to prepare a three-dimensional structure configuration and then performing thermal gelation of the three-dimensional structure configuration. Further provided is a method for preparing said three-dimensional printing composition and a method for preparing a three-dimensional structure using said three-dimensional printing composition.
