The present invention provides a recombinant chicken interleukin-1β protein for producing antibody early and retaining for a longer period of time, which has a sequence of SEQ ID NO:2 or SEQ ID NO:3. The recombinant chicken interleukin-1β protein is created by using point mutation in a genetic engineering method it can significantly improve the original vaccine efficacy to enhance antibody responses, produce antibody one week earlier and extend the protective effect until chickens sold off. Therefore, the recombinant chicken interleukin-1β protein of the present invention can produce significant higher antibody responses than the with-type chicken interleukin-1β protein, it helps to develop avian interleikin-1β vaccine adjuvant and uses in medical application and livestock production.
