THE GOVERNMENT OF THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH & HUMAN SERVICES, THE NATIONAL INSTITUTES OF HEALTH;SWARTZ, KENTON, J.;HACKOS, DAVID, H.
发明人:
SWARTZ, KENTON, J.,HACKOS, DAVID, H.
申请号：
WO2001US03963
公开号：
WO0158952(A3)
申请日:
2001.02.07
申请国别(地区):
世界知识产权组织国际局
年份:
2002
代理人:
摘要：
The present invention provides voltage-gated K<+> channels with amino acid substitutions that produce a constituitively open phenotype when expressed in the cell. The invention includes mutated purified proteins with constituitively open voltage-gated K<+> channel activity, for example those derived from Shaker, Shab, Shal, and Shaw family of voltage-gated K<+> channels. The invention also includes nucleic acid encoding constituitively open voltage-gated K<+> channels, as well as cells and transgenic animals expressing constituitively open voltage-gated K<+> channels. Methods are provided for screening substances for ability to modulate voltage-gated K<+> channels, by exploiting the ability of constituitively active voltage-gated K<+> channels to affect growth or other phenotypic or genotypic characteristics in eukaryotic cells.
