PROBLEM TO BE SOLVED: To provide a preparation method for preparing β-hematin crystals via a heating step, β-hematin crystals obtained by this preparation method, and a vaccine adjuvant composition containing the β-hematin crystals.SOLUTION: The β-hematin crystals have an acicular shape, an average particle size of 0.6-1.2 μm, and characteristic main peaks (any peak including a diffraction angle within ±0.2° thereof) of the diffraction angle 2θ at 7.4°, 12.2°, 21.6°, and 24.1° in X-ray diffraction obtained by powder x-ray diffraction using Cu-Kα radiation.SELECTED DRAWING: NoneCOPYRIGHT: (C)2017,JPO&INPIT