CONWAY, Anthony,JAIN, Sumiti,LEE, Gary K.,REIK, Andreas,TRUONG, Lynn N.
申请号：
SG11202001035T
公开号：
SG11202001035TA
申请日:
2018.08.08
申请国别(地区):
SG
年份:
2020
代理人:
摘要：
Disclosed herein are methods and compositions for engineering cells to express a CAR where the cells also have inactivated TCR, HPRT, PD1, CISH and/or HLA genes, using engineered nucleases comprising at least one DNA binding domain and a cleavage domain or cleavage half-domain in conditions able to preserve cell viability. Polynucleotides encoding nucleases, vectors comprising polynucleotides encoding nucleases and cells comprising polynucleotides encoding nucleases and/or cells comprising nucleases are also provided.
