Mazhuga Aleksandr Georgievich,Мажуга Александр Георгиевич,Nizamov Timur Radikovich,Низамов Тимур Радикович,Uvarova Viktoriya Igorevna,Уварова Виктория Игоревна
申请号：
RU2017146340
公开号：
RU0002656667C1
申请日:
2017.12.27
申请国别(地区):
RU
年份:
2018
代理人:
摘要：
FIELD: chemistry.SUBSTANCE: invention relates to inorganic chemistry and to a method for producing modified crystals of magnetite Fe3O4, containing a fluorescent dye on the surface, which allows visualizing and monitoring their behavior both in a living cell and in a living organism in vivo. Such crystals can be used as drug delivery vehicles and T2-contrast agents. Method for producing modified crystals of magnetite comprises mixing 138 pts.wt. of magnetite crystals and 1 pts.wt. of cholesterol mixture, 1,2-distearoyl-sn-glycero-3-phosphocholine, 1,1-(2-(4-(2-((2-(bis(2-hydroxydodecyl)amino)ethyl)(2-hydroxydecyl)amino)ethyl)piperazin-1-yl)ethylazanediyl)didodecan-2-ol and 1,2-dimyristoyl-sn-glycerymethoxy(polyethylene glycol)-2000, taken in a weight ratio 15:7:75:3, respectively, initially with 60,000 pts. wt. of chloroform, followed by 82,240 pts. wt. of N-methyl-2-pyrrolidone, treatment of the mixture with ultrasound, removing chloroform and adding water thereto, followed by dialysis of the resulting dispersion of the modified crystals of magnetite against water, wherein crystals of magnetite are used as crystals, wherein the crystals of magnetite are obtained by mixing octadecene with iron(III) oleate or iron(III) acetylacetonate, oleic acid and sodium oleate, heating the mixture to 70 °C and its aging at this temperature for 30 minutes, reheating the mixture in an inert gas atmosphere from 70 °C to 320 °C, holding it at this temperature and cooling the mixture to room temperature, carried out in an inert gas atmosphere, introducing into the isopropanol system, separating the crystals of magnetite, dispersing them in a non-polar high boiling organic solvent selected from the group consisting of dibenzyl ether, octadecene and trioctylamine, in the presence of oleic acid and sodium oleate, heating the resulting dispersion to a temperature of 290–350 °C in an inert gas atmosphere, followed by the dropwise addition of a solution of iron(III) oleate to the heated dis
