An electron beam sterilization device includes: a sterilization chamber (6) in which electron beams are applied to containers to perform sterilization; a blocking cover member that covers the sterilization chamber (6) and blocks radiations; and a rotational member having an upper portion exposed through an opening portion (44) formed in the blocking cover member. The blocking cover member includes a fixed blocking cover (41) that covers the upper portion of the rotational member. Between the rotational member and the fixed blocking cover (41), there are provided a rotational annular blocking plate (64) that projects from the rotational member toward the fixed blocking cover (41) and a fixed annular blocking plate (65) that projects from the blocking cover member toward the rotational member. The rotational annular blocking plate (64) and the fixed annular blocking plate (65) face each other with a blocking gap (66) therebetween. The rotational annular blocking plate (64) and the fixed annular blocking plate (65) constitute a blocking lap portion (67) that blocks radiations.