The present invention provides a plant callus tissue extract for hair care, which is extracted from a mixture of lavender callus tissues, Camellia japonica callus tissues, Rosmarinus officinalis callus tissues and Polygonum multiflorum callus tissues via water or organic solvents, wherein the proportion of lavender callus tissues, Camellia japonica callus tissues, Rosmarinus officinalis callus tissues and Polygonum multiflorum callus tissues in the mixture is in the range of 3:3:3:1 to 5:5:2:2. The plant callus tissue extract of the present invention can effectively prevent hair loss, stimulate hair follicle cell growth, have the anti-ultraviolet effect, enhance the formation of melanoma cells and stimulate hair growth.本發明提供一種具有養髮功能的植物癒傷組織萃取物,其係由薰衣草癒傷組織、山茶花癒傷組織、迷迭香癒傷組織及何首烏之癒傷組織之混合物以水或有機溶劑萃取而得,其中該混合物中薰衣草癒傷組織、山茶花癒傷組織、迷迭香癒傷組織及何首烏之癒傷組織的比例在3:3:3:1至5:5:2:2範圍內。本發明之具有養髮功能的植物癒傷組織萃取物係具有減少掉髮、刺激毛囊細胞生長、抗紫外線、增進黑色素細胞以及促進生髮之功效。