A biological frozen device includes: a hollow main body a placing opening configured at one end of the hollow main body to place a bone having malignant osteosarcoma cell into the hollow main body and an intake opening configured at the lateral surface of the hollow main body to enter a low-temperature liquid into the hollow body to lead cryonecrosis of the malignant osteosarcoma tissue of the bone.一種生物性冷凍裝置包含: 一 中空本體;一置入口供一具有惡性骨瘤組織之骨骼置入該中空本體;以及一輸入口,供注入一低溫液體進入該中空本體導致骨骼的惡性骨瘤組織壞死,其位於該中空本體之側面。100‧‧‧生物性冷凍裝置102‧‧‧中空本體102a‧‧‧第一殼體102b‧‧‧第二殼體104‧‧‧置入口106‧‧‧輸入口108‧‧‧排氣口110‧‧‧蓋體110a‧‧‧第一蓋件110b‧‧‧第二蓋件
