A device for securing laboratory animal includes: a hollow body having a room which having an entry end, the entry end located on a end of the hollow body and the entry end can be closed by a closing unit, the other end of the hollow body having a adjusting section, and a through hole set on the hollow body and connected with the room a limiting unit set on the adjusting section of the hollow body and separating a predetermined limiting space from the room.一種實驗動物保定裝置,係包含:一中空體,係具有一腔室,該腔室具一入口端,該入口端係位於該中空體之一端,且該入口端係可以被一封閉件封閉,該中空體另一端具一調整部,該中空體另設一穿孔與該腔室相互連通;及一限位件,係結合於該中空體之調整部,用以將該腔室區隔出一預定大小之限位空間。1...中空體11...腔室12...入口端121...開口122...封閉件13...穿孔14...調整部141...插槽142...開孔15...刻度2...限位件S...限位空間