METHOD FOR DETECTING CONCENTRATION OF ANTIBODIES IN BLOOD SERA TO PATHOGENIC BACTERIA YERSINIA ENTEROCOLITICA OF SEROVARS O:3, O:5 OR O:6.30 AT APPLICATION OF PIEZOGRAVIMETRIC IMMUNOSENSOR
LIPETSKIJ GOSUDARSTVENNYJ TEKHNICHESKIJ UNIVERSITET (LGTU)
发明人:
KALMYKOVA ELENA NIKOLAEVNA,DERGUNOVA ELENA SERGEEVNA,GORSHKOVA RAISA PETROVNA,KOMANDROVA NADEZHDA ALEKSEEVNA,ERMOLAEVA TAT'JANA NIKOLAEVNA
申请号：
RU20050116366
公开号：
RU2288472(C1)
申请日:
2005.05.30
申请国别(地区):
俄罗斯
年份:
2006
代理人:
摘要：
FIELD: immunology. ^ SUBSTANCE: through a flow microcell of 15-20 mcl volume one should pass the flow of phosphate buffer solution-carrier at the rate of about 30-90 mcl/min which should be supplemented with analyzed serumal sample at the volume of 200 mcl. The flow cell is supplied with a horizontally fixed piezogravimetric immunosensor at fluctuation frequency fm and receptor covering based upon one of the LPC Yersinia enterocolitica of serovars O:3, O:5 or O:6.30. Then it is necessary to register the binding LPC Yersinia enterocolitica of serovars O:3, O:5 or O:6.30 with antibodies according to the decrease of sensor's fluctuation frequency from fm up to f to detect the concentration of antibodies according to calibration graph being directly proportional against ▵f being equal to fm-f. Application of the present innovation enables to decrease the procedure of analysis up to 10 min and, also, carry out a re-usable analysis after regeneration of sorbed LPC at sensitivity being 1.3 mcg/ml and detect con
