A fusion protein of porcine reproductive and respiratory syndromevirus (PRRSV) is disclosed. The fusion protein comprises: a) a Pseudomonasexotoxin A (PE) peptide comprising a binding domain and a translocationdomain,located at the N-terminus of the fusion protein； b) a fusion peptide ofAmericanstrain PRRSV ORF6-2~26 (SEQ ID NO: 13) and ORF5-31~63 (SEQ ID NO: 12),or a fusion peptide of European strain PRRSV ORF6-M1~I28 (SEQ10 NO: 14)and ORF5-F31~A64 (SEQ ID NO: 15)； and c) a carboxyl terminal domaincomprising the amino acid sequence of KDEL-KDEL-KDEL located at theC-terminus of the fusion protein, in which the capital letters 'KDEL'represent singleletter amino acid codes, wherein the ORF5-31~63 located between theORF6-2~26 and the carboxyl domain, and the ORF6-F31~A64 is located between theORF6-M1~I28 and the carboxyl domain.
