RECOMBINANT FUSION ANTIGEN GENE, RECOMBINANT FUSION ANTIGEN PROTEIN AND SUBUNIT VACCINE COMPOSITION HAVING THE SAME AGAINST INFECTION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS
The present invention is directed to a recombinant fusion antigen gene, a recombinant fusion antigen protein and a subunit vaccine composition having the same against infection of porcine reproductive and respiratory syndrome virus (PRRSV). A recombinant fusion antigen gene, which encodes glycoprotein GP5 with truncated N’-terminal decoy epitope, a linker sequence and membrane protein M, followed by codon optimization, is expressed by a baculovirus expression system in vitro, thereby enhancing a yield of the recombinant fusion antigen protein. The recombinant fusion antigen protein can be applied in a subunit vaccine composition, for providing vaccinated animals with better protection ability without the risks of virulent spread and virulent recovery.本發明係有關於一種抗豬生殖與呼吸綜合症病毒(PRRSV)感染之重組融合抗原基因、重組融合抗原蛋白及含彼之次單位疫苗組成物,其係將PRRSV之截短N’端誘餌抗原決定位的醣蛋白GP5、連接序列以及膜蛋白M融合為重組基因序列並進行密碼子優化後,所得之重組融合抗原基因利用重組桿狀病毒表現系統進行體外表現,不僅可提升其重組融合抗原蛋白的產量,所得之重組融合抗原蛋白在應用於次單位疫苗組成物時,可提供受免疫動物較佳的疫苗保護力又不具排毒與返毒風險。
