The invention relates to a Japanese encephalitis virus DNA vaccine which includes a virus-like particle expression plasmid of Japanese encephalitis virus and a pharmaceutically acceptable carrier. The virus-like particle expression plasmid of Japanese encephalitis virus includes a cytomegalovirus early gene promoter, a first nucleic acid fragment and a second nucleic acid fragment. Therefore, the Japanese encephalitis virus DNA vaccine is able to induce balanced, high titer and cross-neutralizing antibody against Taiwanese isolates of genotype I and genotype III Japanese encephalitis virus本發明提供一種日本腦炎DNA疫苗，包含日本腦炎病毒類病毒顆粒表現質體和醫藥上可接受載劑，日本腦炎病毒類病毒顆粒表現質體包含巨細胞病毒早期基因啟動子、第一核酸片段以及第二核酸片段。藉此，本發明之日本腦炎DNA疫苗可誘發較平衡且高力價之中和性抗體抵抗台灣本土之第一型及第三型基因型日本腦炎病毒株。
