PROBLEM TO BE SOLVED: To provide a novel vaccine that exerts high effect against not only viruses classified into Japanese encephalitis virus genotype III (GIII) but also viruses classified into genotype (GV).SOLUTION: The present invention provides two-combined Japanese encephalitis virus genotype comprising: (i) Japanese encephalitis virus-like particles containing M protein and E protein derived from a viral classified into Japanese encephalitis virus genotype III; and (ii) Japanese encephalitis virus-like particles containing M protein and E protein derived from a viral classified into Japanese encephalitis virus genotype V.SELECTED DRAWING: None