Cheong Ho CHANG,Hyeong Woo JEONG,Ji Chul YOO,Se Ken YEO,Dong Sam SUH
申请号：
US15527500
公开号：
US20170334969A1
申请日:
2014.12.11
申请国别(地区):
US
年份:
2017
代理人:
摘要：
Disclosed is a method of producing high-concentration collagen for use as a medical material, including: washing tissue of a mammal； subjecting the washed tissue to crushing and immersion in ethyl alcohol； subjecting the tissue to enzymatic treatment with stirring in purified water containing phosphoric acid and pepsin； adding sodium chloride to the collagen subjected to enzymatic treatment, performing stirring, and aggregating collagen； dissolving the aggregated collagen in purified water to give a collagen solution, which is then filtered using a filter and concentrated by removing the pepsin, low-molecular-weight material, and sodium chloride from the collagen solution using a tangential flow filtration device； subjecting the concentrated collagen to sterile filtration, aggregating the collagen using a pH solution in a neutralization tank, and concentrating the collagen by removing a non-aggregated solution； and concentrating the concentrated collagen using a centrifuge and stirring the concentrated collagen using a mixer.
