The present invention relates generally to a method of purifying Factor VII and/or Factor VIIa from a solution containing either or both proteins, the method comprising: diluting the solution containing Factor VII and/or Factor VIIa in a loading buffer； adding the diluted solution from step (i) to a multi-modal anion exchange resinunder conditions selected such that Factor VII and/or Factor VIIa is bound to the resin； optionally washing the resin with a wash buffer； adding an elution buffer to the resin under conditions selected such that Factor VII and/or Factor VIIa is eluted from the resin； and recovering the eluted recombinant Factor VII and/or Factor VIIa； wherein the loading buffer, the optional wash buffer and the elution buffer each comprise about 35 mM or less of calcium ions.
