There is provided an apparatus for washing and disinfecting an endoscope (20) equipped with an insertion tube through which a duct (22a) is formed and a base portion (21) integrated with the insertion tube. The apparatus comprises a bath member (3) providing a washing bath (5) and an accommodating member (10) on which the endoscope is accommodated. The accommodating member is installed in the washing bath. The apparatus further comprises a duct washing unit (16c, 17c, 27, 26, 30, 41-60, 57A, 62-70) and a brush washing unit (26, 41-59, 78, 79, 84, 85). The duct washing unit is equipped with a washing brush (27a) and formed to wash the duct by advancing and retreating the brush through the duct, with the endoscope accommodated on the accommodating member. The brush washing unit is configured to wash the brush by spraying fluid toward the brush when the brush is made to advance and retreat through the duct.