The invention relates to databases of T cell epitopes, especially helper T cellepitopes, for rapid interrogation of protein sequences for the presence of Tcell epitopes. The invention includes full or partial databases and data structuresof T cell epitopes including epitopes identified especially by ex vivo T cellassays with test peptides and includes T cell epitopes identified by extrapolationof data from test peptides. The present invention also includes high throughputmethods for determining the T cell epitope activity of peptides for subsequentinclusion in databases and data structures including methods where subsetsof T cells especially regulatory T cells are removed or inhibited from T cell assaysin order to maximize the sensitivity of detection of T cell epitope activity.
