Methods to prepare a Plant, Plant Cell or plant with a High Content of one or more Proteins, Oils or one or more amino acids with respect to a wild type Plant, Plant Cell or Plant part.Also expression cassettes for the Manipulation of gene expression, and recombinant constructs, Vectors and plants, plant cells or Plant parts that comprise.Plant cells or plants, Parts of plants with a higher content of one or more Proteins, Oils or one or more amino acids so obtained can be useful for the preparation of food products for humans and animals.Plant cells or plants, Parts of plants with a higher content of one or more Proteins, Oils or one or more amino acids so obtained can be useful in Plant Breeding programs to develop other Hybrids or inbred Lines.Claim 1: an expression cassette that confers a higher content of one or more Proteins, Oils or one or more amino acids in a Plant, Plant Cell or Plant part with respect to a wild type Plant, Plant Cell or Plant part of correspondingCharacterized in that it comprises: (a) a promoter is functional in a Plant； (b) a Nucleic Acid Molecule that encodes a POLYPEPTIDE WITH pyruvate kinase activity and it is operationally linked to the heterologous Promoter； and (c) an Intron of Rice, where the Molecule Nucleic Acid comprises: (i) The Nucleotide sequence of SEQ ID no. 1, 3, 5, 7, 9,11 or 13； (ii) a Nucleotide sequence encoding the amino acid sequence of SEQ ID no. 2, 4, 6, 8, 10, 12 and 14； (iii) a Nucleotide sequence that is at least 60% identity with the Nucleotide sequence of SEQ ID no. 1, 3, 5, 7, 9,11 or 13 and encodes a polypeptide that has a Barrel of pyruvate kinase Domain: pf00224 Pfam domain and A / B of pyruvate kinase pf02887 Pfam:； (iv) a Nucleotide sequence encoding an Amino Acid Sequence that is at least 60% identity with the sequence of amino acids Of SEQ ID no. 2, 4, 6, 8, 10,12 or 14 and has a Barrel of pyruvate kinase Domain: pf00224 Pfam domain and A / B of pyruvate kinase pf02887 Pfam:； (V) a Nucleotide seque
