The embodiment of the invention discloses a single-molecule image correction device, and the device comprises a first obtaining module which is used for obtaining a first target binary matrix corresponding to a first image according to a brightness value matrix corresponding to all pixels of the first image, and obtaining a second target binary matrix corresponding to a second image according to a brightness value matrix corresponding to all pixels of the second image; a transformation module which is used for respectively carrying out the two-dimensional discrete Fourier transform of the first and second target binary matrixes, obtaining a first Fourier matrix corresponding to the first target binary matrix, and obtaining a second Fourier matrix corresponding to the second target binary matrix; a second obtaining module which is used for obtaining an offset of the second image relative to the first image according to the first and second Fourier matrixes; and a correcting module which is used for correcting
