The present invention provides a mass-production manufacturing method of saikosaponin for medical use, which can eliminate the defects of the prior art so as to obtain high-purity saikosaponin. The method comprises: preparing a bupleurum drug crude ground object using a first alcohol solvent to extract the bupleurum drug crude ground object and concentrating the same to obtain a bupleurum alcohol extract eluting the bupleurum alcohol extract with an ester solvent until the concentration of a second alcohol is below 5% and eluting with a mixture of ethyl acetate and petroleum ether to obtain a first bupleurum crude extract and a first aqueous layer extract and extracting the first aqueous layer extract with a second alcohol solvent and concentrating and centrifuging the same to obtain a second bupleurum crude extract and a second aqueous layer extract, and further performing vacuum drying on the second bupleurum extract to obtain saikosaponin finished products, wherein each of the first alcohol solvent and the second alcohol solve contains a solution composed by at least one of ethanol, propanol and butanol, and the ester solvent is ethyl acetate.本發明提供一種能夠解決現有技術缺陷可獲得的高純度柴胡皂苷的醫藥用柴胡皂苷之量產製備方法,其包括:準備一柴胡藥材粗粉碎物;以第一醇類溶劑萃取該柴胡藥材粗粉碎物,並經濃縮而得到一柴胡醇類萃取物;將酯類溶劑洗脫該柴胡醇類萃取物,直至該第二醇類濃度在5%以下,並經以乙酸乙酯和石油醚混合液洗脫而獲得一第一柴胡粗萃物以及一第一水層萃取物; 以及以第二醇類溶劑萃取該第一水層萃取物,並經濃縮、離心以獲得一第二柴胡粗萃物以及一第二水層萃取物,再對該第二柴胡粗萃物真空乾燥即得柴胡皂苷成品;其中該第一醇類溶劑、該第二醇類溶劑係分別含有乙醇、丙醇、丁醇中之至少一種所構成的溶液;該酯類溶劑為乙酸乙酯。
