LASSNER MICHAEL,JANTZ DEREK,SMITH JAMES JEFFERSON,CIGAN MARK,FALCO CARL,GAO HUIRONG,LI ZHONGSEN,LIU ZHAN-BIN,SVITASHEV SERGEI
申请号：
ZA201307200
公开号：
ZA201307200B
申请日:
2013.09.26
申请国别(地区):
ZA
年份:
2014
代理人:
摘要：
Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
