LASSNER, MICHAEL,JANTZ, DEREK,SMITH, JAMES JEFFERSON,CIGAN, MARK,FALCO, CARL,GAO, HUIRONG,LI, ZHONGSEN,LIU, ZHAN-BIN,SVITASHEV, SERGEI
申请号：
CA2831144
公开号：
CA2831144A1
申请日:
2012.03.22
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
